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PoC qPCR Test Machines

How It Works

Learn how the PoC qPCR Test Machines work and seamlessly integrate the Cartridge System.

3 Simple Steps

  1. Prep the Cartridge with Sample & Magnetic Beads
  2. Insert into PoC Machine
  3. Select required program and start

Just 2 minutes of manual operation

By using freeze-drying technology, the reagents for nucleic
acid extraction and the PCR reaction are pre-prepared directly in the cartridge.

Manual addition of the reagents is no longer
necessary. The user only needs to add the samples and
magnetic beads. The manual operation time is ≤ 2 min, truly
realizing “samples in, results out”.

Cartridge In - Results Out!

The cartridge consists of four parts: base, reaction chamber, PCR reaction area and top cover. The function relies on the rotation of the bottom piston valve to achieve the liquid transfer between different chambers.

First, add samples and magnetic beads in the sample chamber to perform the lysis and binding steps of the nucleic acid extraction. Then, the nucleic acid is washed and purified three times in the washing chambers and eluted into the elution chamber.

Finally, the reagents are mixed in the PCR reaction area to perform the PCR amplification reaction.

Item Explanation below

  1. Top cover
    Sealing ring - designed to prevent false positive results and environmental contamination caused by aerosol leakage.
  2. Sample Chamber
    Contains sample lysis and binding reagents. Samples and magnetic beads are manually added to perform sample lysis and nucleic acid binding
  3. Washing Chamber 1
    Contains nucleic acid washing reagent. Washing and purifying of the nucleic acid
  4. Washing Chamber 2
    Contains nucleic acid washing reagent. Washing and purifying of the nucleic acid
  5. Washing Chamber 3
    Contains nucleic acid washing reagent. Washing and purifying of the nucleic acid
  6. Elution Chamber
    Contains nucleic acid elution reagents. Elution of nucleic acid adsorbed on magnetic beads
  7. Plunger Activity Chamber
    By pulling the piston in the activity chambers, complete mixing of the reagents and liquids between the chambers is achieved
  8. PCR Reaction Area
    Slice design - can heat up and cool down quickly, which allows PCR amplification to be carried out rapidly and optimal collection of fluorescence signals can be ensured
  9. PCR Reagent Chamber
    Contains the reagents required for the PCR reaction, which are mixed with the elution product to complete the PCR system setup
  10. Base
    Fixed reagent box, including piston valve, for liquid transfer between chambers

Areas of Application

  • CDC
  • Tumor Screening
  • Infection Detection
  • Prenatal Testing
  • Forensic Identification
  • Pet Hospital
  • Food Safety Testing

Flexibility & Expandability

Though small and portable, depending on your needs and requirements, you can choose an expandable number of instruments.

Systems can handle 1, 2 or 4 samples at the same time. Various models can be freely combined, and up to 16 samples can be extracted and tested at the same time.

These operate independently without influencing each other.

4 Fluorescence Detection Channels

Includes 4 fluorescence detection channels, which can achieve multiple detection projects of one sample at the same time. A strict self-checking mechanism ensures the accuracy of the results.

The excitation-emission wavelengths are:

  1. 470-510 nm
  2. 530-565 nm
  3. 585-620 nm
  4. 630-665 nm

Multiple channels can be detected at the same time.

In addition, the instrument has an innovative quantitative detection function that allows the user to freely select the output of qualitative or quantitative results as required.